Optimization of the liquefaction steps of breadfruit starch hydrolysis by alpha-amylase using a statistical approach

Author(s)

I. Pele^1*, M. K. Bolade¹, V. N. Enujiugha¹, D. M. Sanni², A. O. Ogunsua³; ¹Department of Food Science and Technology, Federal University of Technology, Akure, Ondo State, Nigeria; ²Department of Biochemistry, Federal University of Technology, Akure, Ondo State, Nigeria; ³Department of Food Science and Technology, Joseph Ayo Babalola University, Ikeji-Arakeji, Osun State, Nigeria

Abstract

Statistical approach was used to optimize the liquefaction steps of breadfruit starch hydrolysis by alpha-amylase. The optimum condition of breadfruit starch hydrolysis was determined using pure culture of a thermostable alpha-amylase for liquefaction, and the activity of the enzyme determined at varying pH, temperature and time. A 3 x 3 x 3 completely randomized experimental design comprising 3 pH values (pH 6.0, 6.5 and 7.0); 3 temperatures (65, 70 and 75 ⁰C) and 3-time ranges (40, 50 and 60 min) were employed for liquefaction and the data obtained were subjected to multiple regression and the degree of correlation evaluated. The results showed that the p-values of the model terms for sample dry weight, reducing sugar and dextrose equivalent were significant (p < 0.05), however the R2 which was found to be 90.7% for sample dry weight proved suitable for adequate representation of the actual relationship between the selected variables whereas the R2 for reducing sugar and dextrose equivalent which were 43.0 and 32.8% respectively were too low for adequate representation of the actual relationship between the selected variables. The optimal reducing sugar and dextrose equivalent were 14.88% and 12.30 DE, respectively at pH 6.5, 70 ⁰C and 60 min. The model established the actual relationship between the actual variables of the liquefaction and the predictable values of the process while the maltodextrin obtained from the optimized process may serve as a substrate to initiate a saccharification process in the production of glucose syrup.

Keywords

dextrose equivalent; maltodextrin; multiple regression; reducing sugar; sample dry weight

Cite this paper

Pele, I., Bolade, M. K., Enujiugha, V. N., Sanni, D. M., Ogunsua, A. O. (2021), Optimization of the liquefaction steps of breadfruit starch hydrolysis by alpha-amylase using a statistical approach, IRESPUB Journal of Agriculture, Food & Nutrition. Volume 1, Issue 2, Nov-Dec 2021, Page 38-45

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